Key Role for CRB2 in the Maintenance of Apicobasal Polarity in Retinal Pigment Epithelial Cells

  1. Paniagua, Antonio E. 12
  2. Segurado, Alicia 1
  3. Dolón, Jorge F. 1
  4. Esteve-Rudd, Julián 2
  5. Velasco, Almudena 1
  6. Williams, David S. 2
  7. Lillo, Concepción 1
  1. 1 Institute of Neurosciences of Castilla y León, IBSAL, Cell Biology and Pathology, University of Salamanca, Salamanca, Spain
  2. 2 Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States
Journal:
Frontiers in Cell and Developmental Biology

ISSN: 2296-634X

Year of publication: 2021

Volume: 9

Type: Article

DOI: 10.3389/FCELL.2021.701853 GOOGLE SCHOLAR

More publications in: Frontiers in Cell and Developmental Biology

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Cited by

  • Scopus Cited by: 6 (25-11-2023)
  • Web of Science Cited by: 6 (14-10-2023)
  • Dimensions Cited by: 6 (09-03-2023)

JCR (Journal Impact Factor)

  • Year 2021
  • Journal Impact Factor: 6.081
  • Journal Impact Factor without self cites: 5.59
  • Article influence score: 1.282
  • Best Quartile: Q1
  • Area: DEVELOPMENTAL BIOLOGY Quartile: Q1 Rank in area: 6/39 (Ranking edition: SCIE)
  • Area: CELL BIOLOGY Quartile: Q2 Rank in area: 68/195 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2021
  • SJR Journal Impact: 1.44
  • Best Quartile: Q1
  • Area: Cell Biology Quartile: Q1 Rank in area: 72/296
  • Area: Developmental Biology Quartile: Q1 Rank in area: 15/82

Scopus CiteScore

  • Year 2021
  • CiteScore of the Journal : 3.5
  • Area: Developmental Biology Percentile: 36
  • Area: Cell Biology Percentile: 23

Journal Citation Indicator (JCI)

  • Year 2021
  • Journal Citation Indicator (JCI): 0.85
  • Best Quartile: Q2
  • Area: CELL BIOLOGY Quartile: Q2 Rank in area: 70/204
  • Area: DEVELOPMENTAL BIOLOGY Quartile: Q2 Rank in area: 11/40

Dimensions

(Data updated as of 09-03-2023)
  • Total citations: 6
  • Recent citations: 6
  • Relative Citation Ratio (RCR): 0.6
  • Field Citation Ratio (FCR): 3.5

Abstract

Apicobasal polarity is essential for epithelial cell function, yet the roles of different proteins in its completion is not fully understood. Here, we have studied the role of the polarity protein, CRB2, in human retinal pigment epithelial (RPE) cells during polarization in vitro, and in mature murine RPE cells in vivo. After establishing a simplified protocol for the culture of human fetal RPE cells, we studied the temporal sequence of the expression and localization of polarity and cell junction proteins during polarization in these epithelial cells. We found that CRB2 plays a key role in tight junction maintenance as well as in cell cycle arrest. In addition, our studies in vivo show that the knockdown of CRB2 in the RPE affects to the distribution of different apical polarity proteins and results in perturbed retinal homeostasis, manifested by the invasion of activated microglial cells into the subretinal space. Together our results demonstrate that CRB2 is a key protein for the development and maintenance of a polarized epithelium.

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