Phosphate solubilizing rhizobia originating from Medicago, Melilotus and Trigonella grown in a Spanish soil

  1. Villar-Igea, M. 1
  2. Velázquez, E. 1
  3. Rivas, R. 1
  4. Willems, A. 2
  5. van Berkum, P 3
  6. Trujillo, M. E. 1
  7. Mateos, P. F. 1
  8. Gillis, M. 2
  9. Martínez-Molina, E. 1
  1. 1 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

  2. 2 Ghent University
    info

    Ghent University

    Gante, Bélgica

    ROR https://ror.org/00cv9y106

  3. 3 U.S. Department of Agriculture, ARS, Soybean Genomics and Improvement Laboratory, Beltsville, MD, 20705, USA
Actas:
First International Meeting on Microbial Phosphate Solubilization

Año de publicación: 2007

Páginas: 149-156

Congreso: First International Meeting on Microbial Phosphate Solubilization,Salamanca, Spain, July 16–19, 2002

Tipo: Aportación congreso

DOI: 10.1007/978-1-4020-5765-6_22 GOOGLE SCHOLAR

Resumen

Although phosphate solubilization is a character known to be present in species of Mesorhizobium, this property has not been described before in species of Sinorhizobium. The type strains of the three species that nodulate Medicago species, Sinorhizobium meliloti, S. medicae and Rhizobium mongolense, do not solubilize phosphate from bicalcium phosphate in plate culture. We observed phosphate solubilization among isolates we obtained from nodules of Medicago sativa, Melilotus and Trigonella growing in a Spanish soil. Phenotypic and genetic analyses of these isolates led to the conclusion that they were placed within the genus Sinorhizobium with characteristics in common with S. meliloti and S. medicae. The group of strains solubilizing phosphate is distinguishable to strains from S. meliloti and S. medicae basing on LMW RNA profiles, TP-RAPD patterns and SDS-PAGE profiles.

Referencias bibliográficas

  • Bergersen F J 1961 The growth of Rhizobium in synthetic media. Aust. J. Biol. Sci. 14, 349–360.
  • de Lajudie P, Willems A, Pot B, Dewettinck D, Maestrojuan G, Neyra M, Collins MD, Dreyfus B, Kersters K and Gillis M 1994 Polyphasic taxonomy of Rhizobia: Enmendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov. Int. J. Syst. Bacteriol. 44, 715–733.
  • Halder A K, Mishra A K and Chakrabartty P K 1990 Solubilization of phosphatic compounds by Rhizobium. Indian J. Microbiol. 30, 311–314.
  • Kumar S, Tamura K, Jakobsen I B and Nei M 2001 Molecular Evolutionary Genetics Analysis Software. Arizona State University, Tempe, Arizona. USA.
  • Laemmli U K 1970 Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680–685.
  • Nicholas K B and Nicholas H B 1997. Genedoc: A tool for editing and annotating multiple sequence alignments. Multiple Sequence Alignment Editor and Shading Utility, 2.6.001. Distributed by the authors.
  • Peix A, Rivas-Boyero A A, Mateos P F, Rodríguez-Barrueco C, Martínez-Molina E and Velázquez E 2001 Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol. Biochem. 33, 103–110.
  • Rigaud J and Puppo 1975 Indole-3-acetic catabolism by soybean bacteroids. J. Gen. Microbiol. 88, 223–228.
  • Rivas R, Velázquez E, Valverde A, Mateos P F and Martínez-Molina E 2001 A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species. Electrophoresis 22, 1086–1089.
  • Rivas R, Velázquez E, Palomo J L, Mateos P, García-Benavides P and Martínez-Molina E 2002a Rapid identification of Clavibacter michiganensis subspecies sepedonicus using two primers random amplified polymorphic DNA (TP-RAPD) fingerprints. Eur. J. Plant Pathol. 108, 179–184.
  • Rivas R, Velázquez E, Willems A, Vizcaíno N, Subba-Rao N S, Mateos P F, Gillis M, Dazzo F B and Martínez-Molina E 2002b A new species of Devosia that forms a nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L. f.) Druce. Appl. Environ. Microbiol. 68, 5217–5222.
  • Rome S, Fernández Brunel M P B, Normand P and Cleyet-Marel J C 1996 Sinorhizobium medicae sp. nov., isolated from annual Medicago spp. Int. J. Syst. Bacteriol. 46, 972–980.
  • Rodríguez H and Fraga R 1999 Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech. Adv. 17, 319–339.
  • van Berkum P 1990 Evidence for a third uptake hydrogenase phenotype among the soybean bradyrhizobia. Appl. Environ. Microbiol. 56, 3835–3841.
  • van Berkum P, Beyene D and Eardly B D 1996 Phylogenetic relationships among Rhizobium species nodulating the common bean Phaseolus vulgaris L. Int. J. Syst. Bacteriol. 46, 240–244.
  • van Berkum P, Beyene D, Bao G, Campbell T A and Eardly B D 1998 Rhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbioses with Medicago ruthenica [(L.) Ledebour]. Int. J. Syst. Bacteriol. 48, 13–22.
  • van Berkum P and Fuhrmann J J 2000 Evolutionary relationships among the soybean bradyrhizobia reconstructed from 16S rRNA gene and Internally Transcribed Spacer region sequence divergence. Int. J. Syst. Evol. Microbiol. 50, 2165–2172.
  • Vincent J M 1970 The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of Root-Nodule. Ed. J M Vincent. pp. 1–13. Blackwell Scientific Publications, Oxford.