Different effects on pulmonary VEGF expression andseptation in newborn rats with prenataldexamethasone and betamethasone

  1. A Remesal 1
  2. L San Feliciano 1
  3. M Isidoro 1
  4. D Ludeña 1
  5. C Sanz 1
  1. 1 Universidad de Salamanca

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

Acta Paediatrica

ISSN: 1651-2227

Year of publication: 2009

Volume: 98

Issue: s460

Pages: 218-219

Congress: Annual Meeting of the European Society for Paediatric Research

Type: Conference paper


JCR (Journal Impact Factor)

  • Year 2009
  • Journal Impact Factor: 1.768
  • Journal Impact Factor without self cites: 1.595
  • Article influence score: 0.643
  • Best Quartile: Q2
  • Area: PEDIATRICS Quartile: Q2 Rank in area: 34/94 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2009
  • SJR Journal Impact: 0.822
  • Best Quartile: Q1
  • Area: Medicine (miscellaneous) Quartile: Q1 Rank in area: 631/2938
  • Area: Pediatrics, Perinatology and Child Health Quartile: Q1 Rank in area: 60/284


Betamethasone might be a better choice than dexameth-asone for antenatal therapy. Vascular endothelial growthfactor (VEGF) is critical for normal lung development. Theaim is to evaluate differences between dexamethasone andbetamethasone in the expression of VEGF and its reper-cussions in septation during rat lung development underoxidative stress.Methods: Dexamethasone or betamethasone wereadministered to gestant Wistar rats. Newborn rats wererandomized to breathe room air (A), hypoxia 2 hours +hyperoxia 2 hours (HH) or hypoxia 2 hours + air (HA).We evaluated pulmonary VEGF in rats at 4 days of life byinmuno-histochemistry (sc-152, Santa Cruz) and mRNAVEGF by RT-PCR. The VEGF inmuno-staining and themRNA VEGF were quantified in the pulmonary paren-chyma with ImageJ software. Morphometric assessmentwas done on coded slides with 200x magnification and aeye piece with a sample grid pattern. Differences betweenthe groups were determined by one way ANOVA (p< 0.05).Results are shown in Table 1. Conclusions: Prenatal dexamethasone and betametha-sone have different actions on pulmonary VEGF expressionwith repercussion in the alveolarization. Antenatal dexa-methasone was associated with a significantly lower expres-sion of pulmonary VEGF with a correlation in the decrease ofthe alveolarization, when compared with betamethasone.