Associacion Of 308G>A And -238G>A TNF-α Polymorphisms With Asthma In A Spanish Population

  1. Batista, J.C. 1
  2. Padrón, J.R. 1
  3. Isidoro-García, M. 1
  4. Sanz, C.S. 1
  5. García, V. 1
  6. Benito, D. 1
  7. Hernández, L. 1
  8. Dávila, I.J. 1
  9. Lorente, F. 1
  1. 1 Hospital Universitario de Salamanca
    info

    Hospital Universitario de Salamanca

    Salamanca, España

    ROR https://ror.org/0131vfw26

Journal:
Journal of Allergy and Clinical Immunology

ISSN: 0091-6749

Year of publication: 2010

Volume: 125

Issue: 2

Pages: AB50

Type: Article

DOI: 10.1016/J.JACI.2009.12.229 GOOGLE SCHOLAR

More publications in: Journal of Allergy and Clinical Immunology

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Abstract

RATIONALE: Tumor necrosis factor (TNFα) is a pro-inflammatory cytokine that plays an important role in the pathogenesis of asthma. TNFα gene promoter polymorphisms -308G>A and -238G>A seem to be involved in the production of protein at the transcriptional level. We have examined the association of both polymorphisms with the presence of asthma in a Spanish population. METHODS: We analyzed 216 patients diagnosed of asthma according to GINA guidelines and 157 non-asthmatic, non-atopic controls. DNA was extracted from peripheral blood using an inmunomagnetic procedure. Genotyping was performed using site-specific PCR method. The SPSS software, version 17.0 and the Shesis software platform were employed for statistical analyses. RESULTS: We found an association between the presence of asthma and the polymorphism -308G>A of TNFα. The A allele was more frequent in the group of patients (Fisher's p value of 0.016). The SNP 238G>A did not show a significant association. However, the haplotype analysis showed an association with asthma phenotype. The AG (-308G>A and -238G>A) combination was more common in asthma (Fisher's p value of 0.017 O.R. [95%CI] = 1.911 [1.113-3.282]) but no association was observed for the AA haplotype. The Hardy-Weinberg Equilibrium was observed in all cases. CONCLUSIONS: The polymorphism -308G>A of TNFα is related to the asthma phenotype in our population. This association could be due to a modification in the expression of TNFα. This association could be modified by the combination with alleles of other SNPs. Haplotype analysis provide a more complete information regarding genetic association.