363C>T polymorphism of TPSAB1 genein allergic patients

  1. Hernandez-Hernandez, L 1
  2. Garcia-Isidoro, M 1
  3. Pascual, M 1
  4. Sanz, C 1
  5. Moreno, E 1
  6. Davila, I 1
  7. Lorente, F 1
  1. 1 Hospital Universitario de Salamanca
    info

    Hospital Universitario de Salamanca

    Salamanca, España

    ROR https://ror.org/0131vfw26

Actas:
Allergy

ISSN: 0105-4538 1398-9995

Año de publicación: 2011

Volumen: 66

Número: s94

Páginas: 485-485

Congreso: EAACI Congress of the European Academy of Allergy and Clinical Immunology

Tipo: Aportación congreso

Resumen

Background:Tryptase is a serine proteasestored in the secretion granules of mastcells. The mayor types of tryptase arebeta-tryptase and alpha-tryptase, bothcoded by the TPSAB1 gene. Several SNPshave been described along this gene. The363C>T SNP is recognized as an EcoRVRFLP and distinguishes alpha and betaallele of TPSAB1 gene. The alpha allelehas been previously related to protectionin allergy.Method:We have studied 125 atopicpatients and 133 non-atopic control indi-viduals with normal serum tryptase levels.DNA fragments were amplified by PCRusing primers corresponding to TPSAB1gene and incubated with the EcoRVenzyme. Subsequently the fragments werevisualized in an agarose gel. Frequencieswere determined and compared with thosedescribed in the literature.Result:In the global population, the allelicfrequency of alpha allele was 0.62 whilebeta allele frequency was 0.38. The genoti-pic frequency of beta-beta genotype was0.76 and 0.24 was to alpha-beta genotypefor the RFLP analysis. There were no sig-nificant differences in the allelic and geno-typic distribution between controls andatopic patients although the alpha allelepresence was slightly higher in the controlgroup. Statistical significant associationbetween SNP 363C>T (RFLP of EcoRV)and clinic-biological characteristics, IgElevels, tryptase levels and positive skin teststo aeroallergens, dander, dust mites, moldsand pollens was not observed. Tryptaselevels in carriers of alpha allele were4.77lg/l (2.33 of standard deviation)ver-sus6.15lg/l (5.99 of standard deviation) innon-carriers individuals (P=0.057).Conclusion:Although the alpha allele wasslightly more frequent in our control popu-lation, we did not find the previouslydescribe protective role of this allele in ourpopulation of allergic patients.