Genetic variants regulate the promoteractivity of PTGDR
- García-Sánchez, A 1
- Marcos-Vadillo, E 2
- Sanz, C 1
- García-Solaesa, V 2
- Moreno-Rodilla, E 12
- Muñoz-Bellido, F 2
- Macias, E 2
- Isidoro-García, M 12
- Dávila, I 12
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1
Universidad de Salamanca
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2
Hospital Universitario de Salamanca
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ISSN: 0105-4538, 1398-9995
Year of publication: 2015
Volume: 70
Issue: s101
Pages: 161-161
Congress: European Academy of Allergy and Clinical Immunology Congress
Type: Conference paper
Abstract
Background:PTGDR, a receptor ofPGD2, has been proposed as a candidategene in allergy. The objective of this workwas to interrogate the contribution ofPTGDR SNPs to PTGDR expression incell culture.Method:Four PTGDR reporter constructsof 700 bp were created by PCR amplifica-tion of genomic DNA from homozygoussubjects who had the following alternativehaplotypes (positions -613, -549, -441 and -197): CTCT, CCCT, TCCT and CCCC.The fragments were cloned in the pGL3-basic vector. The cloned regions were con-firmed by sequencing.Results:The haplotype with the CTCTsequence (-613C, -549T, -441C, and -197T)was significantly associated with the lowestreporter activity, while the rest of haplo-types, CCCC, CCCT and TCCT, showed aremarkable reporter activity increase of 3magnitude orders. CTCT: 0.0 0.0;CCCC: 3.11 0.17; CCCT: 3.06 0.17;TCCT: 3.08 0.24 logRLU, (P-value<0.0001. Conclusion:The findings presented in thisstudy suggest a differential PTGDR pro-moter activity according to genetic vari-ants. Response to new treatments based onPTGDR modulation could depend on thegenetic background in allergic patients