Nitro-fatty acids modulate germination onset through S-nitrosothiol metabolism

  1. Mata-Pérez, Capilla 12
  2. Begara-Morales, Juan C 1
  3. Padilla, María N 1
  4. Chaki, Mounira 1
  5. Sánchez-Calvo, Beatriz 33
  6. Carreras, Alfonso 1
  7. Aranda-Caño, Lorena 1
  8. Melguizo, Manuel 1
  9. Valderrama, Raquel 1
  10. Sánchez-Vicente, Inmaculada 2
  11. Lorenzo, Óscar 2
  12. Barroso, Juan B 1
  1. 1 Universidad de Jaén
    info

    Universidad de Jaén

    Jaén, España

    ROR https://ror.org/0122p5f64

  2. 2 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

  3. 3 Universidad de la República
    info

    Universidad de la República

    Montevideo, Uruguay

    ROR https://ror.org/030bbe882

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Journal:
Plant Physiology

ISSN: 0032-0889,  1532-2548

Year of publication: 2025

Volume: 197

Issue: 2

Type: Article

DOI: 10.1093/PLPHYS/KIAF038 GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Plant Physiology

Related Projects

Los ácidos grasos nitrados como nuevos mediadores de la germinación en plantas (2024-2025)

Abstract

Nitro-fatty acids (NO2-FAs) have emerged as key components of nitric oxide (NO) signaling in eukaryotes. We previously described how nitro-linolenic acid (NO2-Ln), the major NO2-FA detected in plants, regulates S-nitrosoglutathione (GSNO) levels in Arabidopsis (Arabidopsis thaliana). However, the underlying molecular mechanisms remain undefined. Here, we used a combination of physiological, biochemical, and molecular approaches to provide evidence that NO2-Ln modulates S-nitrosothiol (SNO) content through S-nitrosylation of S-nitrosoglutathione reductase1 (GSNOR1) and its impact on germination onset. The aer mutant (a knockout mutant of the alkenal reductase enzyme; AER) exhibits higher NO2-Ln content and lower GSNOR1 transcript levels, reflected by higher SNO content and S-nitrosylated proteins. Given its capacity to release NO, NO2-Ln mediates the S-nitrosylation of GSNOR1, demonstrating that NO2-FAs can indirectly modulate total SNO content in plants. Moreover, the ectopic application of NO2-Ln to dormant seeds enhances germination success similarly to the aer germination rate, which is mediated by the degradation of master regulator ABSCISIC ACID INSENSITIVE 5 (ABI5). Our results establish that NO2-FAs regulate plant development through NO and SNO metabolism and reveal a role of NO2-FAs in plant physiology.

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Funding information

Funders

  • ERDF
  • Spanish Ministry of Science, Innovation and Universities
    • PID2022-142973NB-I00
    • PID2020-117774GA-I00
  • Junta de Andalucía
  • Recalibration of the Spanish University System

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