Characterization of two PP2Cs-D interactors during germination and seedling development of Arabidopsis thaliana

  1. Fresnillo Herrero, Patricia
Supervised by:
  1. Óscar Lorenzo Sánchez Director
  2. María Dolores Rodríguez Martín Co-director

Defence university: Universidad de Salamanca

Fecha de defensa: 22 July 2016

  1. Juan Carlos del Pozo Benito Chair
  2. Mónica Balsera Secretary
  3. Kazumi Nakabayashi Committee member

Type: Thesis


Phosphoregulation is one of the most important postranslational mechanisms in plants. Among plant protein phosphatases (PPs) the largest family is formed by PP type-2C (PP2Cs), that dephosphorylates target proteins usually contributing to their deactivation and playing a role both in hormone signalling and plant development. PP2Cs best characterized clade, cluster A, has been related with abscisic acid (ABA) signalling pathway acting as negative regulators. FsPP2C2, from Fagus sylvatica, belongs to cluster D PP2Cs, a less characterized clade, and was isolated by our research group as responsive to ABA during beechnuts germination. In order to determine its interacting partners, a Y2H screening was carried out with FsPP2C2 as bait and an Arabidopsis cDNA library as prey. These thesis comprehends the characterization of 3 of these interactions during germination and early development.