Caracterización y separación de liposomas por microencapsulación

  1. SANCHEZ-CARPINTERO, María José 1
  2. SANCHEZ-NAVARRO, Amparo 1
  3. DE JESUS-VALLE, María José 2
  1. 1 Departamento de Farmacia y Tecnología Farmacéutica. Facultad de Farmacia
  2. 2 Departamento de Farmacia y Tecnología Farmacéutica. Facultad de Farmacia (Salamanca).
Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2016

Volume: 1

Issue: 1

Pages: 93-100

Type: Article

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Abstract

Liposomes are microscopic vesicles composed of concentric lipid bilayers entrapping aqueous compartments. Proteins and lipids are among biocompatible and biodegradable materials used for microencapsulation, being albumin an interesting protein due to its role as a drugs carrier.The aim of the study was to encapsulate ciprofloxacin loaded liposomes into Albusomes® a novel pharmaceutical vehicle based on albumin coated liposomes.The vesicle preparation was carried out using the lipid hydration method and sonication method using a mixture of egg phosphatidylcholine (EPC), diethyldodeccyiamonium (DDA) and cholesterol (Ch).The liposomes were encapsulated with bovine serum albumin by induced flocculation. The pellet, containing the albumin coated liposomes with ciprofloxacin trapped into water vesicle core and albumin coating layers, was isolated by centrifugation. Concentration of ciprofloxacin was quantified in the supernatant and the absence of lipid vesicles was confirmed. The proposed vehicle (Albusomes®) showed to be able to encapsulate liposomes with a high drug entrapment efficacy (86.0 ± 5.0% and 93.52±3.94%). for lipid hydration method and sonication method, respectively.

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