Estudio de genes implicados en la formación de biofilms con potencial implicación en la patogenia bacteriana utilizando librerías de mutantes Tn5.

  1. GALÁN, Irene 1
  2. GARCÍA-FRAILE, Paula 1
  3. RIVAS, Raúl 1
  1. 1 Departamento de Microbiología y Genética, laboratorio 210. Edificio Departamental de Biología. Campus Miguel de Unamuno. Universidad de Salamanca
Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2018

Volume: 3

Issue: 1

Pages: 67-76

Type: Article

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Abstract

Biofilm formation is important in pathogenesis of a big number of bacteria. Escherichia coli, is a gram- negative bacterium, rod-shaped, facultatively anaerobic, coliform bacterium commonly found in the intestine of many animal species. Most E. coli strains are non- pathogenic, but some serotypes can cause serious diseases in their hosts. E.coli biofilm development is a complex process important for disease and engineering applications. Therefore, the description of genes implicated in the biofilm formation process is of utmost importance. Consequently, in this study we have grown a collection of 40 different mutants from the DH5? E.coli strain by using the Tn5 transposon insertion, in order to study the possible implications of the mutated genes in the biofilm formation process in this bacterium. The discovery of genes implicated in biofilm formation in E.coli may serve in future investigations of drugs against infections caused by this bacterium.

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