Silenciamiento de la glucosa-6-fosfato deshidrogenasa (G6PDH) en células de carcinoma de riñón humano HEK-293T, mediante la técnica del ARN de interferencia

  1. GUTIERREZ, Beatriz
  2. BOLAÑOS, Juan Pedro
Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2016

Volume: 1

Issue: 1

Pages: 73-83

Type: Article

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Abstract

The role of pentose-phosphate pathway and its involvement in glycolysis is nowadays still unknown. Recent findings have shown a direct relationship between the over-expression of this pathway and the uncontrolled proliferation of differents malign tumours. Clarifying the the importance of this pathway has therefore become an important key in the fight against cancer. This study has silenced the gene of the enzyme which catalyses the first reaction of this pathway, glucose-6-phosphate dehydrogenase, by RNA interference in human renal carcinoma cells HEK-293T. The effect of silencing this gene was controlled by measuring lactate (?mol/mg protein)/24h, using cells with the luciferase gen silenced as the control group. The results from this study show an important decrease in the production of lactate in cells with the silenced enzyme: 2,73 ?mol/mg protein/24h compared to 7,81 ?mol/mg protein/24h in the control group. This decrease clearly shows how the pentose-phosphate pathway highly contributes to the production of pyruvate through the glycolysis.   

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