Detección de Rickettsia spp. en garrapatas fiadas en personas en Castilla y León

  1. HERNÁNDEZ, Luis
  2. VIERA, Carmen
  3. MURO, Antonio 1
  1. 1 Departamento Biología animal, Parasitología, Ecología, Edafología y Química agrícola. Facultad de Farmacia. Universidad de Salamanca
Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2020

Volume: 5

Issue: 1

Pages: 37-45

Type: Article

DOI: 10.14201/FJ2020513745 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Farmajournal

Abstract

Ticks are hematophagous parasites of terrestrial vertebrates with great importance because they are transmitters of pathogenic agents. The aim of this study consisted of the analysis of ticks fixed on people in Castilla y Leon in summer, more specifically the detection of rickettsiosis. We recived 709 ticks removed to people in the diferents health center or hospital in this community. In the laboratory was carried out its identification and extraction of DNA by a commercial kit Genomic DNA from Tissue. Then, we performed an individualized PCR to each sample in order to know if they are carriers of Rickettsia spp. To see the results of the PCR, was made an electrophoresis gel agarose, which is displayed with a transilluminator BioDoc-it2 Imaging Systems. Finally, the positive products were purified and quantified. Of the samples analyzed, for genes gltA and OmpA, were obtained 67 positive (9.44%), presenting greater prevalence in the provinces of Avila and Burgos. Therefore, this study concludes that the growing interest in tick-borne diseases is indisputable, and the data obtained are very important in guiding measures to control and prevent these diseases.

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