Interacciones entre activadores y represores en el inicio de la transcripción en Saccharomyces cerevisiae

Résumé

Autosomic dominant lateral temporal epilepsy (ADLTE) is a form of partial epilepsy with secondary generalized seizures, caused by mutations in LGI1. This gene was initially identified in 1998, as a tumour suppressor in multiform glioblastoma, affecting to the malignancy of these tumours. In silico analysis of the primary sequence of LGI1, revealed four paralogues of the gene, which shared a very similar domain structure and were named as LGI1, LGI2, LGI3 and LGI4. The existence of similar types of epilepsy with common features for that seen for ADLTE, suggests that some other members of this gene family could be involved in these pathologies. Moreover, the presence of four paralogues, makes the idea of a certain degree of functional redundancy, possible. This fact, could be explained by the complementary distribution of cells expressing the genes of this family in the brain. In this work, we show a detailed expression analysis of the mRNA of the members of the LGI family in adult mouse brain. The distribution of these genes is regionally heterogeneous, suggesting that since their origin as a common ancestral gene, a subfunctionalization might have occurred. In this work, we try to shed light on these possible functions.