Aplicación del método Strong-LAMP para la detección molecular de Strongyloides stercoralis en una población de Porto Iguazú, Misiones, Argentina.

  1. Rodríguez Diego, Carmen 1
  2. Fernández-Soto, Pedro 1
  3. Muro Álvarez, Antonio 1
  1. 1 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

Revue:
Farmajournal

ISSN: 2445-1355

Année de publication: 2020

Volumen: 5

Número: 2

Pages: 73-82

Type: Article

DOI: 10.14201/FJ2020527382 DIALNET GOOGLE SCHOLAR lock_openAccès ouvert editor

D'autres publications dans: Farmajournal

Résumé

Strongiloides stercoralis is a nematode globally distributed and the chief causative agent of human Strongyloidiasis. Chronic infection is clinically asymptomatic, but, it can cause Hyperinfection Syndrome in immunocompromised patients. At present, the lack of a standardized diagnosis generates an underestimation of the prevalence of the disease. Coprological test, considered as the gold-standard, has a very low sensitivity. An alternative is the specific and sensitive LAMP method for the detection of S.stercoralis, the Strong-LAMP. In the present study, it have been used a total of 100  stool samples collected from a population of Porto Iguazú, Misiones (Argentina) and stored on filter paper until analysis. When Strong-LAMP was applied for the detection of Strongyloides spp. DNA, up to 39 samples (39%) were positive, including 5 positive obtained by coprological test, compared to 7 positive samples (7%) obtained by parasitological test. Therefore, Strong-LAMP has demonstrated more sensitive method for the detection of Strongyloides stercoralis in stool samples stored in filter paper than parasitological diagnostic methods and could be used in combination with parasitological methods in endemic areas to establish with greater reliability the real prevalence of the disease.

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