Determination of cellular DNA content of Iberian salamanders by flow cytometry

  1. Orfao, Alberto 1
  2. Martín-Sánchez, Roberto 2
  3. Lizana, Miguel 3
  4. Ciudad, Juana 4
  5. Márquez, Rafael 5
  6. López, Antonio 6
  1. 1 6Servicio de Citometría, Universidad de Salamanca, Spain
  2. 2 2Departamento de Biología Animal, Universidad de Salamanca, 37071 Salamanca, Spain; Centro de Biología Ambiental, Faculdade de Cíências, Universidade de Lisboa, Campo Grande, Lísboa, P-1700, Portugal
  3. 3 1Departamento de Biología Animal, Universidad de Salamanca, 37071 Salamanca, Spain
  4. 4 4Servicio de Citometría, Universidad de Salamanca, Spain
  5. 5 3Museo Nacional de Ciencias Naturales, José Gutierrez Abascal
  6. 6 5Servicio de Citometría, Universidad de Salamanca, Spain
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Revue:
Amphibia-Reptilia

ISSN: 0173-5373,  1568-5381

Année de publication: 2000

Volumen: 21

Número: 4

Pages: 411-418

Type: Article

DOI: 10.1163/156853800300059304 GOOGLE SCHOLAR lock_openAccès ouvert editor

D'autres publications dans: Amphibia-Reptilia

Résumé

The DNA content per diploid nucleus (2 C-value) was determined for the eight species of Iberian salamanders by flow cytometry. All species showed high values, which are characteristic of Caudata. The species with the lowest value was Pleurodeles waltl (48.25 pg DNA/cell) and Salamandra salamandra had the highest values (70.55 pg DNA/cell). There were no statistical differences between sexes in any of the eight species and DNA content was not directly related with phylogeny. The values obtained are often higher than previously published estimates obtained with older and potentially less accurate methodologies. We discuss the possible relationship between cellular DNA content and reproductive biology