P193 Chemical organization of the pcd mouse brain: calcium-binding proteins, cholinergic and nitrergic systems
- Alonso, J.
- Weruaga, E.
- Muñoz del Rey, J.R.
- Arévalo, R.
- Alcalde, I.
- Alonso, J.R.
- Porteros, A.
- Valero, J.
ISSN: 0953-6205
Año de publicación: 2003
Volumen: 14
Páginas: S86
Tipo: Artículo
Otras publicaciones en: European Journal of Internal Medicine
Resumen
Purkinje cell degeneration (pcd) is an autosomal mutation located on mouse chromosome 13. Pcd mutant mice are interesting neurodegeneration models since they suffer a postnatal, progressive and natural degeneration of specific neuronal populations throughout the brain. In addition to the early moderate cerebellar ataxia, there are slow progressive degenerations of olfactory mitral cells, photoreceptors and other neuronal populations. These degenerative processes take place at differentpace and elicit different kind of responses. The pcd mouse offers a unique opportunity to evaluate brain changes after cell-specific loss. We present an interactive atlas detailing the chemo-architecture of the pcd mouse brain. The atlas includes corona1 sections of analogue rostro-caudal levels from both control and mutant brains, stained for Nissl, acetylcholinesterase, NADPH-diaphorase, parvalbumin, calbindin D-28k, and calretinin. These techniques label (with the exception of Nissl) specific neuronal populations and are valuable neuroanatomical tools since they provide frequently ‘Golgi-like’ stainings. This atlas shows digital microscopical pictures, at different magnifications, in an easy way to compare the same field and staining between pcd mutants and control mice. Supported by grants of DGES, Plan National sobre las Drogas (Ministerio de1 Interior), Fundacion Samuel Solorzano and Junta de Castilla y Leon.