Aplicación de la técnica LAMP para la detección de Loa loa y Mansonella perstans

  1. Gallego, Gloria 1
  2. Febrer-Sendra, Begoña 1
  3. Crego-Vicente, Beatriz 1
  4. García-Bernalt Diego, Juan 1
  5. Fernández-Soto, Pedro 1
  1. 1 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2021

Volume: 6

Issue: 1

Pages: 53-63

Type: Article

DOI: 10.14201/FJ2021615363 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Farmajournal

Abstract

Filariases are endemic diseases of tropical regions caused by filiform nematodes transmitted by insect bites. They produce high morbidity. Loiasis (Loa Loa) and mansonellosis (Mansonella perstans) globally affect 10 and 100 million people, respectively. The diagnosis of certainty is parasitological, but it does not detect early infections or low microfilaeremia, and it is necessary to consider the periodicity of microphilaremias in blood. Molecular techniques, such as PCR, have great sensitivity and specificity, but they are expensive, technically complex and require infrastructure not available in endemic areas of scarce resources. LAMP technology (loop-mediated isothermal amplification) has advantages over PCR as faster, scarce equipment, more tolerant of inhibitors and the results can be observed colorimetrically. In this work, LAMP technology is applied and evaluated for the detection of DNA from Loa loa and M. perstans in 22 blood samples stored on filter paper from individuals living in Equatorial Guinea. The samples were analyzed microscopically, using qPCR and LAMP. The molecular methods were more sensitive than microscopy. LAMP resulted more sensitive than qPCR for the detection of DNA from Loa loa and M. perstans.

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