Changes in Gene Expression Associated with Collagen Regeneration and Remodeling of Extracellular Matrix after Percutaneous Electrolysis on Collagenase-Induced Achilles Tendinopathy in an Experimental Animal Model: A Pilot Study

  1. Calderón-Díez, Laura
  2. Arias-Buría, José L.
  3. Méndez-Sánchez, Roberto
  4. Herrero-Turrión, Javier
  5. Sánchez-Sánchez, José Luis
  6. Fernández-de-las-Peñas, César
  1. 1 Department of Physical Therapy, Universidad de Salamanca, 37007 Salamanca, Spain
  2. 2 Physical Therapy Department, Mutua Accidentes Laborales, FREMAP, 37007 Salamanca, Spain
  3. 3 Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, 37007 Salamanca, Spain
  4. 4 Instituto Investigación Biomédica de Salamanca (IBSAL), Universidad de Salamanca, 37007 Salamanca, Spain
  5. 5 Department of Physical Therapy, Occupational Therapy, Physical Medicine and Rehabilitation, Universidad Rey Juan Carlos (URJC), Alcorcón, 28922 Madrid, Spain
  6. 6 Cátedra Institucional en Docencia, Clínica e Investigación en Fisioterapia: Terapia Manual, Punción Seca y Ejercicio Terapéutico, Universidad Rey Juan Carlos, Alcorcón, 28922 Madrid, Spain
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Revista:
Journal of Clinical Medicine

ISSN: 2077-0383

Año de publicación: 2020

Volumen: 9

Número: 10

Páginas: 3316

Tipo: Artículo

DOI: 10.3390/JCM9103316 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Clinical Medicine

Resumen

Percutaneous electrolysis is an emerging intervention proposed for the management of tendinopathies. Tendon pathology is characterized by a significant cell response to injury and gene expression. No study investigating changes in expression of those genes associated with collagen regeneration and remodeling of extracellular matrix has been conducted. The aim of this pilot study was to investigate gene expression changes after the application of percutaneous electrolysis on experimentally induced Achilles tendinopathy with collagenase injection in an animal model. Fifteen Sprague Dawley male rats were randomly divided into three different groups (no treatment vs. percutaneous electrolysis vs. needling). Achilles tendinopathy was experimentally induced with a single bolus of collagenase injection. Interventions consisted of 3 sessions (one per week) of percutaneous electrolysis or just needling. The rats were euthanized, and molecular expression of genes involved in tendon repair and remodeling, e.g., Cox2, Mmp2, Mmp9, Col1a1, Col3a1, Vegf and Scx, was examined at 28 days after injury. Histological tissue changes were determined with hematoxylin–eosin and safranin O analyses. The images of hematoxylin–eosin and Safranin O tissue images revealed that collagenase injection induced histological changes compatible with a tendinopathy. No further histological changes were observed after the application of percutaneous electrolysis or needling. A significant increase in molecular expression of Cox2, Mmp9 and Vegf genes was observed in Achilles tendons treated with percutaneous electrolysis to a greater extent than after just needling. The expression of Mmp2, Col1a1, Col3a1, or Scx genes also increased, but did not reach statistical significance. This animal study demonstrated that percutaneous electrolysis applied on an experimentally induced Achilles tendinopathy model could increase the expression of some genes associated with collagen regeneration and remodeling of extracellular matrix. The observed gene overexpression was higher with percutaneous electrolysis than with just needling.

Referencias bibliográficas

  • 10.1111/j.1600-0838.2012.01509.x
  • 10.1016/j.fas.2011.04.007
  • 10.1136/bjsports-2015-095422
  • 10.1007/s11999-008-0286-4
  • 10.1302/2058-5241.2.160075
  • 10.1136/bjsports-2019-100608
  • 10.1371/journal.pone.0122220
  • 10.1111/evj.12035
  • 10.1002/art.21672
  • 10.1016/S0945-053X(01)00128-7
  • 10.1136/bjsm.2008.050740
  • 10.1002/14651858.CD010960.pub2
  • 10.5792/ksrr.16.055
  • 10.1136/bjsports-2018-099934
  • Sanchez-Ibáñez, (2009)
  • 10.1186/s40634-016-0070-4
  • 10.1177/1071100718754421
  • 10.3390/jcm9072068
  • 10.1016/j.jpain.2018.04.017
  • 10.3390/jcm9061837
  • Medina-Mirapeix, (2019), Rev. Fisioter. Invasiva, 2, pp. 91
  • García-Vidal, (2019), Rev. Fisioter. Invasiva, 2, pp. 80
  • 10.1055/s-0039-3401880
  • 10.1055/s-0040-1712511
  • 10.1371/journal.pone.0192769
  • 10.1016/0003-2697(87)90021-2
  • 10.1186/1471-2164-15-874
  • 10.1038/nprot.2008.73
  • 10.1038/s41598-017-05466-y
  • 10.1371/journal.pone.0161590
  • 10.1371/journal.pone.0185282
  • 10.1016/S0736-0266(02)00064-5
  • 10.1177/03635465000280040901
  • 10.1093/rheumatology/keg448
  • 10.1186/1471-2474-12-86
  • 10.1007/s11999-008-0255-y
  • 10.1097/00003086-200207000-00022
  • 10.1007/s00167-003-0391-6
  • 10.3109/03008207.2013.787418
  • 10.1002/jor.22575
  • 10.1152/japplphysiol.91563.2008
  • 10.1007/s00441-014-1834-8
  • Sharma, (2006), J. Musculoskelet. Neuronal Interact., 6, pp. 181
  • 10.1002/jor.22624
  • 10.1016/S0363-5023(03)00366-6
  • 10.1002/jor.20769
  • 10.1146/annurev-bioeng-071811-150122
  • 10.1016/j.jhsa.2008.07.003
  • 10.1371/journal.pone.0097453