Evaluation of biofungicide activity of endophytic bacteria isolated from blackberry bush (Rubus ulmifolius Schott) against two phytopathogenic fungi

  1. Rocio Roca Couso 1
  2. Paula García Fraile 1
  3. Jose David Flores Felix 2
  4. Raul Rivas Gonzalez 1
  1. 1 Universidad de Salamanca

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

  2. 2 Universidade da Beira Interior

    Universidade da Beira Interior

    Covilhã, Portugal

    ROR https://ror.org/03nf36p02

1st International Electronic Conference on Agronomy

Editorial: MDPI

Ano de publicación: 2021

Tipo: Achega congreso


Agriculture is one of the main human activities, supplying food for a human population which is continuously growing. Crop diseases are responsible for huge economic losses and they are caused for different types of organisms such as fungi. Botrytis cinerea and Fusarium sp. are two of the most dangerous due to their ability to expand and colonize different crops. Pesticides have been used to control them, but the overuse has resulted in the development of resistances. Thus, biopesticides have risen as a potential alternative against these phytopathogens since their use decrease the negative effects and increase the positive responses by the plants.The main objective of this work is the isolation of endophytic bacteria from blackberry bush and the evaluation of their activity against both B. cinerea and Fusarium sp.Firstly, we evaluated different mechanisms in vitro. The production of enzymes with hydrolytic activities against fungal cell wall components and the production of diffusible and volatile organic compounds (VOCs) with antifungal activity. Results showed 54% of isolates produced diffusible molecules against B. cinerea and 51% against Fusarium sp. While 19% produced VOCs against B. cinerea and 16% against Fusarium sp.Secondly, genomes of selected strains were sequencing and studied to find those molecules which are responsible for the activities seen in vitro. Operons involved in biosynthesis of molecules such as PKS (polyketide synthases), NRPS (NonRibosomal Peptide Synthetase) and phenolic compounds, known for their antifungal activity, were found their genomes.In conclusion, selected strains isolated from blackberry bush may represent a strong ally against some of the worst pathogens that agriculture faces, such as Botrytis cinerea and Fusarium sp.Authors thank the Ministerio de Ciencia e Innovación for financing the project “Análisis de la biodiversidad funcional con aplicación para la mejora en la producción de arándano y mora”. Ref.: PID2019-109960RB-100.