Brassica napus Endophytes Have the Potential to Protect the Crop against the Blackleg Disease Causal Agent, the Fungus Leptosphaeria maculans

  1. Peral-Aranega, Ezequiel 1
  2. Saati-Santamaría, Zaki 12
  3. Rivas, Raúl 123
  4. García-Fraile, Paula 123
  1. 1 Microbiology and Genetics Department, University of Salamanca
  2. 2 Spanish-Portuguese Institute for Agricultural Research (CIALE)
  3. 3 Associated Research Unit of Plant-Microorganism Interaction (University of Salamanca-IRNASA-CSIC)
Proceedings of The 1st International Electronic Conference on Plant Science

Year of publication: 2020

Type: Conference paper

DOI: 10.3390/IECPS2020-08758 GOOGLE SCHOLAR


According to the UN, the World population is going to achieve 8.000 million people imminently [1]. Finding a sustainable and efficient way to feed the growing population is one of the major challenges of this generation.Crop yields can be promoted by plant growth promoting (PGP) bacteria in substitution of chemical fertilization, which has shown to cause a wide list of environmental harms. On the other side, pest control with chemical biocides is not only harmful for the environment but could also be a direct cause of human disease. For this reason, BCA (biological control agents) are being studied. Microorganisms can aid crops against the attack of phytopathogens, by direct antagonism (growth inhibition, p.e.) or by the early activation of the plant defense mechanisms –priming- [2].In this study, 49 Brassica napus bacterial endophytes were tested in vitro as antagonists of the fungus Leptosphaeria maculans, the causal agent of the most damaging disease of this crop [3]. The screening allowed us to find 13 endophytes with high in vitro antifungal potential. Two Pseudomonas strains, belonging to the species P. brassicacearum and P. orientalis, respectively, greatly inhibited the fungus. Genomes sequences of these strains were sequenced, and their genetic potential to produce secondary metabolites was analyzed on the antiSMASH platform (v5.1.2). Both strains genome analyses showed diverse biosynthetic gene clusters (BGCs) that may be implicated in the production of novel secondary metabolites that might be implicated in the antifungal activity, according to its relative similarity to other BGCs in the platform database.

Bibliographic References

  • UN Department of Economic and Social Affairs. Available online: (Accessed on 07/09/2020).
  • García-Fraile, P.; Menéndez, E.; Rivas, R. Role of bacterial biofertilizers in agriculture and forestry. AIMS Bioeng. 2015, 2, 183–205, doi:10.3934/bioeng.2015.3.183.
  • Marcroft, S.J.; Sosnowski, M.R.; Scott, E.S.; Ramsey, M.D.; Salisbury, P.A.; Howlett, B.J. Brassica napus plants infected by Leptosphaeria maculans after the third to fifth leaf growth stage in south-eastern Australia do not develop blackleg stem canker. Eur. J. Plant Pathol. 2005, 112, 289–292, doi:10.1007/s10658-005-1050-1.