The TCCTG Haplotype (-613C>T, -549T>C, -441C>T, -197T>C and -95G>T) of PTGDR Gene is Associated with Allergic Sensitization
- Davila, I.J. 1
- Sanz, C. 1
- Isidoro-Garcia, M. 1
- Garcia-Solaesa, V. 1
- Benito-Pescador, D. 1
- Hernandez-Hernandez, L. 1
- Moreno, E. 1
- Muñoz-Bellido, F.J. 1
- Laffond, E. 1
- Lorente, F. 1
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1
Hospital Universitario de Salamanca
info
ISSN: 0091-6749
Year of publication: 2011
Volume: 127
Issue: 2
Pages: AB5-AB5
Type: Article
More publications in: Journal of Allergy and Clinical Immunology
Abstract
RATIONALE: PTGDR gene has been associated with asthma susceptibility by several groups, including ours. In addition we have observed a stronger association of PTGDR with allergic asthma. In the present study we have evaluated the possible association of PTGDR gene with allergen sensitization. METHODS: In this study, 550 individuals (299 atopic patients and 251 non-atopic non-asthmatic controls). Skin prick tests with a battery of common aeroallergens were performed to both patients and controls. After PCR and direct sequencing haplotypic combinations of -613C>T, -549T>C, -441C>T, -197T>C and the non-previously described -95G>T SNPs of PTGDR were analyzed. Assessment of haplotypic interactions were performed with the Shesis software platform. EMQN guidelines were observed in all laboratory procedures. RESULTS: We found that the TCCTG (-613T, -549C, -441C, -197T and -95G) haplotype was more frequent in mite allergic patients (Fisher p=0.05), pollen allergic patients (Fisher p=0.026; OR:1.72[1.06-2.79]) and particularly in patients sensitized to both pollen and mites (Fisher p=0.005; OR:2.39[1.28-4.46] than in controls. CONCLUSIONS: The TCCTG haplotype was significantly associated with pollen and mite allergy. Interestingly, the CCT (-549C, -441C, -197T) haplotype has been previously described to be a high transcriptional efficiency haplotype which could determine a high PTGDR expression. In the present study we described an expanded combination of PTGDR SNPs associated with allergen sensitization.