The TCCTG Haplotype (-613C>T, -549T>C, -441C>T, -197T>C and -95G>T) of PTGDR Gene is Associated with Allergic Sensitization

  1. Davila, I.J. 1
  2. Sanz, C. 1
  3. Isidoro-Garcia, M. 1
  4. Garcia-Solaesa, V. 1
  5. Benito-Pescador, D. 1
  6. Hernandez-Hernandez, L. 1
  7. Moreno, E. 1
  8. Muñoz-Bellido, F.J. 1
  9. Laffond, E. 1
  10. Lorente, F. 1
  1. 1 Hospital Universitario de Salamanca
    info

    Hospital Universitario de Salamanca

    Salamanca, España

    ROR https://ror.org/0131vfw26

Revista:
Journal of Allergy and Clinical Immunology

ISSN: 0091-6749

Año de publicación: 2011

Volumen: 127

Número: 2

Páginas: AB5-AB5

Tipo: Artículo

DOI: 10.1016/J.JACI.2010.12.034 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Allergy and Clinical Immunology

Resumen

RATIONALE: PTGDR gene has been associated with asthma susceptibility by several groups, including ours. In addition we have observed a stronger association of PTGDR with allergic asthma. In the present study we have evaluated the possible association of PTGDR gene with allergen sensitization. METHODS: In this study, 550 individuals (299 atopic patients and 251 non-atopic non-asthmatic controls). Skin prick tests with a battery of common aeroallergens were performed to both patients and controls. After PCR and direct sequencing haplotypic combinations of -613C>T, -549T>C, -441C>T, -197T>C and the non-previously described -95G>T SNPs of PTGDR were analyzed. Assessment of haplotypic interactions were performed with the Shesis software platform. EMQN guidelines were observed in all laboratory procedures. RESULTS: We found that the TCCTG (-613T, -549C, -441C, -197T and -95G) haplotype was more frequent in mite allergic patients (Fisher p=0.05), pollen allergic patients (Fisher p=0.026; OR:1.72[1.06-2.79]) and particularly in patients sensitized to both pollen and mites (Fisher p=0.005; OR:2.39[1.28-4.46] than in controls. CONCLUSIONS: The TCCTG haplotype was significantly associated with pollen and mite allergy. Interestingly, the CCT (-549C, -441C, -197T) haplotype has been previously described to be a high transcriptional efficiency haplotype which could determine a high PTGDR expression. In the present study we described an expanded combination of PTGDR SNPs associated with allergen sensitization.