Role of platelet C3G in vesicle trafficking and spreading. Involvement in hemostasis and platelet-mediated inflammation

  1. Fernández Infante, Cristina
Supervised by:
  1. María Carmen Guerrero Arroyo Director

Defence university: Universidad de Salamanca

Fecha de defensa: 26 May 2023

Committee:
  1. Consuelo González Manchón Chair
  2. Miguel Vicente Manzanares Secretary
  3. Ignacio Rubio Revuelta Committee member
Department:
  1. MEDICINA

Type: Thesis

Abstract

In this work we present evidence of a role for C3G in the modulation of platelet spreading through the regulation of lamellipodia formation via its participation in the Rac1/WAVE2/Arp2/3 pathway, which is independent of its GEF function. In addition, C3G would regulate the secretion of α-granules by modulating the formation of the trans-SNARE and the VARP/VAMP-7/Arp2/3 complexes, as well as the activation of Ral, in a Rap1-dependent manner. Finally, we present preliminary results, both in vitro and in vivo, on a novel role of the C3G protein in the platelet-mediated inflammatory response, through the regulation of PLA formation, with NE and B lymphocytes, and the release of inflammatory factor.