Efecto del ácido oleico sobre la comunicación intercelular en astrocitos de rata durante el desarrollo

  1. LAVADO, E.
  2. SÁNCHEZ-ABARCA, L.
  3. TABERNERO, A.
  4. BOLAÑOS, J.P.
  5. MEDINA, J.M.
Journal:
Ars pharmaceutica

ISSN: 2340-9894,  0004-2927

Year of publication: 1996

Volume: 37

Issue: 4

Pages: 739-751

Type: Article

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Abstract

The role of oleic acid in the modulation of gap junction permeability was studied in cultured rat astrocytes by the scrape-Ioading/Lucifer yellow transfer technique. lncubation with oleic acid caused a dose-dependent inhibition of gap junction permeability by 79.5% at 50 IlM and no further inhibition was observed by increasing the oleic acid concentration up to 100 1lM. The oleic acid-mediated inhibition of gap junction permeability was reversible and was prevented by bovine serum albumin (BSA). The potency of oleic acid-related compounds in inhibiting gap junction permeability was arachidonic acid > oleic acid > oleyl alcohol > palmitoleic acid > stearic acid > octanol > caprylic acid > palmitic acid > methyl-oleyl estero Oleic acid and arachidonic acid, but not methyl-oleyl ester, increased glucose uptake by astrocytes. Our results support the hypothesis that the inhibition of gap junction permeability is associated with the increase in glucose uptake. We suggest that iduring the perinatal period, oleic acid may be a physiological mediator in astrocyte differentiation by regulating intercellular communication.

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