Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt

  1. Jin, Xue 1
  2. Bai, Yang 1
  3. Khashi u Rahman, Muhammad 1
  4. Kang, Xiaojun 3
  5. Pan, Kai 1
  6. Wu, Fengzhi 1
  7. Pommier, Thomas 4
  8. Zhou, Xingang 1
  9. Wei, Zhong 2
  1. 1 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Department of Horticulture Northeast Agricultural University Harbin China
  2. 2 Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Laboratory of Bio‐interactions and Crop Health, National Engineering Research Center for Organic‐based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing Agricultural University Nanjing China
  3. 3 Department of Plant & Microbial Biology University of Minnesota Saint Paul Minnesota USA
  4. 4 Univ Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne Villeurbanne France
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Revista:
iMeta

ISSN: 2770-596X,  2770-596X

Año de publicación: 2022

Volumen: 1

Número: 3

Tipo: Artículo

DOI: 10.1002/IMT2.37 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: iMeta

Resumen

Biochar amendment is acknowledged to favor plant resistance against soil-borne diseases. Although plant-beneficial bacteria enrichment in the rhizosphere is often proposed to be associated with this protection, the mechanism behind this stimulating effect remains unelucidated. Here, we tested whether biochar promotes plants to recruit beneficial bacteria to the rhizosphere, and thus develop a disease-suppressive rhizosphere microbiome. In a pot experiment, biochar amendment decreased tomato Fusarium wilt disease severity. Using a transplanting rhizosphere microbiome experiment, we showed that biochar enhanced the suppressiveness of tomato rhizosphere microbiome against Fusarium wilt disease. High-throughput sequencing of 16S ribosomal RNA gene and in vitro cultures further indicated that the recruited suppressive rhizosphere microbiome was associated with the increase of plant-beneficial bacteria, such as Pseudomonas sp. This amendment also enhanced the in vitro chemoattraction and biofilm promotion activity of tomato root exudates. Collectively, our results demonstrate that biochar amendment induces tomato seedlings to efficiently recruit a disease-suppressive rhizosphere microbiome against Fusarium wilt.

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