Función biológica y regulación de la ciclina específica de meiosis Rem1 en Schizosaccharomyces Pombe
- Malapeira Argilaga, Jordi
- José Ayté del Olmo Director
Defence university: Universitat Pompeu Fabra
Fecha de defensa: 04 April 2007
- Martí Aldea Malo Chair
- Josep Vilardell Secretary
- Cristina Martín Castellanos Committee member
- Rosa Aligué Alemany Committee member
- Diego Haro Bautista Committee member
Type: Thesis
Abstract
The main goal of this doctoral thesis is the characterization of the meiotic cyclin Rem1, in Schizosaccharomyces pombe. First of all we analized the transcription and expresion profiles of rem1 during meiosis. We observed the maximum of expresion during meiosi I and the kinase activity of the complex cyclin-Cdk had exactly the same profile. Then, we analized the transcription profile of the mature mRNA of rem1, showing that this transcription depends on Mei4 through the FLEX boxes which are loclized in the rem1 promoter. An antisense RNA was also detected at the begining of the meiosis. We next diceded to study the function of this cyclin. Firstly we observed that overexpression of Rem1 is toxic for the cell during mitotic cell growth. Moreover, a function for Rem1 was observed during meiosis I. Rem1 is also required in order to obtain normal levels of meiotic intragenic recombination, but it is not necesary for the intergenic recombination. Finally, we could detect a genetic interaction betwen Rem1 and the meiotic S phase cyclin, Cig2.