Changes in hairless rhino-j mice (hr-rh-j) COLON

  1. I. de San José 2
  2. N. García 2
  3. R. Cabo 2
  4. S. de la Fuente 2
  5. J.A. Vega 1
  6. J. Represa 2
  1. 1 Universidad de Valladolid
    info

    Universidad de Valladolid

    Valladolid, España

    ROR https://ror.org/01fvbaw18

  2. 2 Universidad de Oviedo
    info

    Universidad de Oviedo

    Oviedo, España

    ROR https://ror.org/006gksa02

Actas:
XVIII Congress of the Spanish Anatomical Society

ISSN: 1136-4890,  2340-311X

Año de publicación: 1999

Volumen: 3

Número: S1

Páginas: 30

Congreso: XVIII Congress of the Spanish Anatomical Society

Tipo: Aportación congreso

GOOGLE SCHOLAR lock_openAcceso abierto editor

Resumen

A mutation in the hr gene is responsible for typical epithelium phenotype in hairless mice. As this gene is expressed at high level not only in the skin but also in the large intestine, the aim of the study was to clarify its role in the colon. We have analysed by morphological (hae- matoxiline-eosine and Pas stain) and immunohystochemi- cal techniques (chromogranin A) the colon of a mutated mouse strain, the hairless (hr-rh-j) type carrying the homozygotous hr gene rhino mutation.The colon was studied in young (3 months, n= 3) and adult (9 months, n=7) wild type and mutated mice.Major structural changes were found in the adult-ani- mal groups revealing a significant increase in the size of villi, as well as in the number of globe cells respect to their normal littermates.Chromogranin A immunohystochemistry revealed a significant increase in the expression of this protein in certain parts of colon in older mutated animals in compa- rison with wild type.CONCLUSION: These results suggest that hr gene is involved in the structural maintenance of the mature colon, rather than in development. Our findings may also be consistent with an accelerated aging of this animals which is also present in other body structures.