Determinación de la composición fenólica en granos ancestrales de uso alimentario. Comparación de métodos analíticos y de actividad antioxidante

  1. M.ª Delcourt-garcía, Magdalena 1
  2. Escribano-Bailón, María Teresa 1
  3. Dueñas, Montserrat 1
  1. 1 Universidad de Salamanca
    info

    Universidad de Salamanca

    Salamanca, España

    ROR https://ror.org/02f40zc51

Journal:
Farmajournal

ISSN: 2445-1355

Year of publication: 2020

Volume: 5

Issue: 2

Pages: 27-41

Type: Article

DOI: 10.14201/FJ2020522741 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Farmajournal

Abstract

The aim of this study was to quantify the total phenolic compounds of different ancestral grains such as two varieties of tef (Eragrostis tef) white and brown, buchwheat (Fagopyrum esculentum) and spelta (Triticum spelta), using different spectrophotometric methods (Folin-Ciocalteu and Fast Blue BB) and to compare with the obtained results using high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). Furthermore, antioxidant activity of the extracts from these samples has been compared using two different in vitro assays: ferric reducing power (FRAP) and the ability to scavenge the ABTS•+. The use of HPLC-DAD-MS allowed us to detect different groups of phenolic compounds: hydroxybenzoic and hydroxycinnamic derivatives, flavonols, flavones and flavanols. Flavones derivatives were the most abundant in spelta, white and brown teff. However, buckwheat presented the highest in flavanols (64 %). The phenolic compounds quantification carried out by HPLC-DAD-MS could be more appropriate than the obtained results using spectrophotometric methods. Regarding the antioxidant activity, the buckwheat seeds showed the highest value, which could be related to the highest phenolic content.

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